Cphycocyanin cpc is a phycobiliprotein found in blue green algae, such as spirulina platensis, is often used as a dietary nutritional supplement and exhibit a variety of pharmacological properties. Optimization of phycocyanin extraction from spirulina platensis using factorial design. It is evident from the data presented in table 1 that the maximum dry weight 0. Please login to display the rating of all cosmetics. Research article phycocyanin extraction from spirulina platensis and extract stability under various ph and temperature. Spirulina platensis and its contents have been linked to a nutritional component in. Home personal carecosmetics single extracts spirulina spirulina platensis extract. Medical application of spirulina platensis derived c. C phycocyanin cpc is a phycobiliprotein found in blue green algae, such as spirulina platensis, is often used as a dietary nutritional supplement and exhibit a variety of pharmacological properties. A wide range of phycocyanin extraction methods were studied for the processing of. Phycocyanin, a blue pigment, is a type of phycobiliproteins. Chlorella vulgaris and spirulina platensis showed higher protein contents of spirulina as it reached 64%wwso,it may be used in agriculture as a nitrogen biofertilizer and as an animal and fish growth promoter. Cphycocyanin extraction from spirulina platensis oven.
The current work reports protein extraction from spirulina platensis cyanobacterial biomass in order to simultaneously generate a potential coproduct and a biofuel feedstock with reduced nitrogen. Effect of ph on phytochemical and antibacterial activities. Original article phyocyanin extraction from microalgae spirulina platensis assisted by ultrasound irradiation. Cultivation of spirulina arthrospira platensis in low. The commercial production of spirulina has long been implemented in ponds, but photobioreactors and other innovating culture techniques tend to develop. The effects of solidliquid ratio, extraction temperature and extraction time on cphycocyanin concentration, extract purity and yield of extraction were considered to optimize conditions for phycocyanin extraction. Extraction and purification of cphycocyanin from dry. In the present study the growth of two species of spirulina, viz. Chemical composition and potential application of spirulina. Spirulina is one of the oldest life appearances on earth. Buy spirulina arthrospira platensis liquid extract 4 oz on. Therefore phycocyanin extraction from fresh biomass was studied. Effect of ph on phytochemical and antibacterial activities of spirulina platensis 343 figure 1. A simple method for efficient extraction and purification of cphycocyanin from spirulina platensis geitler.
Spirulina spirulina platensis extract bio botanica. Phycocyanin is an important commercially available blue food colorant. Comparison of different extraction methods for phycocyanin. Spirulina platensis extract ingredient for cosmetics formulations find latest products launched and their applications in relevant industry news and technical articles. Cultivation of spirulina arthrospira platensis in low cost. Cyanobacteria spirulina platensis basic protein cphycocyanin.
Cultivation of two species of spirulina spirulina platensis and spirulina platensis var lonar on sea water medium and extraction of c phycocyanin murugan t. More recently, special attention has been given to s. Bio chemical analysis of spirulina biomass showed presence of 17 amino acids,10%ww carbohydrates,8%wwfibers and 8%wwlipids. Extraction and separation of phycocyanin from spirulina. Spirulina platensis ccc540 was procured from the culture collection of centre for conservation and utilization of blue green algae ccubga, iari, new delhi, india. Because of its various potential properties, phycocyanin is applied to various fields, such as nutraceutical, pharmaceutical, medicine, cosmetics, and biotechnological research. Spirulina liquid extract, organic spirulina arthrospira platensis. A method for stably purifying a functional dye, phycocyanin from spirulina platensis was developed by a hexane extraction process combined with high pressure. Cphycocyanin extraction from spirulina platensis wet. Also, another study suggested that aqueous extract of s.
Spirulina platensis is a nontoxic cyanobacterium characterized by high levels of carbonate and bicarbonate in an alkaline environment. Help pages, faqs, uniprotkb manual, documents, news archive and biocuration projects. The best growth of spirulina platensis found was at 320c and grew well from 30. Cphycocyanin extraction from spirulina platensis wet biomass 47 brazilian journal of chemical engineering vol. Cphycocyanin is a natural blue dye used in food and pharmaceutical industry. One of its species spirulina platensis or its extract showed therapeutic. Spirulina platensis protects against renal injury in rats with gentamicininduced acute tubular necrosis. The effects of solidliquid ratio, extraction temperature and extraction time on c phycocyanin concentration, extract purity and yield of extraction were considered to optimize conditions for phycocyanin extraction. The cost and application of phycocyanin are highly dependent on its purity index. Stable isolation of phycocyanin from spirulina platensis associated with highpressure extraction process. Spirulina platensis arthrospira physiology, cellbiology and.
Spirulina and its components have been shown to have positive benefits across a range of human health indications from malnutrition to antioxidant properties. The sonication method using an ultrasonic processor was conducted for 5, 12. Arthrospira platensis, also called blue algae, and commonly known as spirulina, is very interesting for its high content of pc. Material and methods culture conditions 1 spirulina platensis leb52 strain was obtained from the oceanographic institute, university of sao paulo and kept in the laboratory of biochemical engineering of the federal university of the rio grande as leb52. Spirulina, a filamentous cyanobacterium, botanists classify it as a micro alga belonging to cyanophyceae, it has a simple structure but a complex composition.
Screening of cyanobacterial strain showed maximum phycobiliprotein in spirulina platensis. Phycocyanin extraction from spirulina platensis and. Materials and methods microorganism spirulina platensis ln1 was an old isolate from the lonar soda crater, bhuldhana, maharashtra. Efficacy of different extraction methods of phycocyanin from. In the present study, a simple and efficient method to extract cphycocyanin from spirulina platensis wet biomass is reported. Techniques and equipment used in planting, processing machinery cost, etc introduction to spirulina. Chlorophyll production from spirulina platensis single. This was necessary because this dye is known to be very unstable during normal extraction processes. Nutritional and toxicological aspects of nutr hosp.
Buy spirulina liquid extract, organic spirulina arthrospira platensis tincture 4oz on. Extraction of phycocyanin from spirulina plantesis using. Herein we report an optimization study of various phycocyanin extraction methods from spirulina platensis cyanobacterium biomass dry, frozen and wet. Extraction and separation of phycocyanin from spirulina using. Cphycocyanin extraction from spirulina platensis wet biomass.
Spirulina is a biomass of cyanobacteria bluegreen algae that can be consumed by humans and animals. Spirulina platensis extract cosmetic ingredient inci. Longterm effect of spirulina platensis extract on dmbainduced hamster buccal pouch carcinogenesis immunohistochemical study. The pharmacological actions, such as antiinflammation, antioxidation, antitumor, immunological enhancement, and hepatorenal protection of c phycocyanin cpc from spirulina platensis, have been reported, and cpc has important value of development and utilization either as drug or as functional food. Phyocyanin extraction from microalgae spirulina platensis assisted by ultrasound irradiation. The interaction of znii ions with cyanobacteria spirulina platensis basic protein cphycocyanin cpc is studied by fluorescence spectroscopy. Characterization of the lipid fraction of spirulina platensis. The mobility of psi and pq molecules in spirulina platensis cells during state transition. Pdf optimization of protein extraction from spirulina. The effects of temperature and biomasssolvent ratio on phycocyanin concentration and extract purity were evaluated to determine the optimum conditions for phycocyanin extraction.
Effect of ph on phytochemical and antibacterial activities of. Review article medical application of spirulina platensis. Cultivation of spirulina arthrospira platensis in low cost seawater based medium for extraction of value added pigments. The analysis of the effects of the ffd allowed defining gelation conditions of protein concentrate from spirulina platensis and the gel was submitted to evaluation of viscosity profile as a function of temperature. Sivasankari, naganandhini and david ravindran department of biology, gandhigram rural institutedeemed university, dindigul, tamilnadu, india corresponding author abstract introduction spirulina is a bluegreen algae owing to the.
The binding energy of znii ions with cphycocyanin is determined using equilibrium dialysis and atomic absorption spectroscopy. Review article medical application of spirulina platensis derived c phycocyanin qianliu, 1 yinghonghuang, 1 ronghuazhang, 1 tiangecai, 2 andyucai 1,3 college of pharmacy, jinan university, guangzhou, china. Quantitative analysis of cphycocyanin from spirulina. The effects of temperature and biomasssolvent ratio on phycocyanin concentration and extract purity were evaluated to determine the. A number of drying methods studied for the processing of spirulina oven dried and sun dried resulted in loss of phycocyanin. The extractions were carried out using six different methods, including chemical organic and inorganic acid treatment, physical freezing and thawing, sonication, homogenization. The extraction yield of the phycocyanin from the three different methods was estimated by the procedure below. Today, let us go through spirulina extraction process. Jul 16, 2014 spirulina platensis ccc540 was procured from the culture collection of centre for conservation and utilization of blue green algae ccubga, iari, new delhi, india. Spirulina health benefits theres many health benefits associated with spirulina because of the nutrients found in spirulina such as protein, betacarotene, antioxidants, phycocyanin, chlorophyll, and other vitamins and minerals. Arthrospira platensis, also known as spirulina, is a gram negative, nontoxic species of cyanobacteria with a wide array of uses in the natural and commercial world. Comparison of different extraction methods for phycocyanin extraction and yield from spirulina platensis s. Cold water extracts from spirulina platensis were prepared as follows.
Phycocyanin and phycocyanobilin from spirulina platensis. Its inclusion with the phylum cyanobacteria as seen in figure 2 indicates that a. Spirulina arthrospira platensis liquid extract 4 oz. In this study, ammonium chloride is presented as a novel, effective, and. Xifeng zhang 1, fenqin zhang 1, guanghong luo 2, shenghui yang 2, danxia wang 2. Spirulina platensis considering the extraction yield. Nutritional and toxicological aspects of spirulina. Antioxidant and anticancer activity of spirulina platensis. Cphycocyanin extraction from spirulina platensis ovendried. Lea vernes, philippe granvillain, farid chemat, maryline vian. Silveira st1, burkert jf, costa ja, burkert ca, kalil sj.
Among many of spirulina bioactive components, blue protein cphycocyanin and. Efficacy of different extraction methods of phycocyanin. Effect of ph values on growth of spirulina platensis even 30 days of incubation. Mccarty,3 masakazu fujii,1 noriko ikeda,1 kunihisa kobayashi,1 noriyuki sonoda,1,2 and ryoichi takayanagi1 1department of medicine and bioregulatory science, graduate school of medical sciences. Phycocyanin and phycocyanobilin from spirulina platensis protect against diabetic nephropathy by inhibiting oxidative stress jing zheng,1 toyoshi inoguchi,1,2 shuji sasaki,1 yasutaka maeda,1 mark f. In the current study, a simple efficient method for extracting pc from spirulina platensis powder is described. Spirulina platensis is a cyanobacterium that has been largely studied due to its commercial importance as a source of protein, vitamins, essential amino acids, and fatty acids2,3. S botany department, university of madras, tamilnadu, india. Considering the uses of c phycocyanin, the aim of this study was to evaluate some methods previously reported to extract c phycocyanin and other bioproducts, in order to find the best procedure to extract c phycocyanin from wet biomass of spirulina platensis considering the extraction yield. Extraction and purification of cphycocyanin from spirulina.
Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1. Pdf phycocyanin extraction from spirulina platensis and. In this work, an exhaustive 9 characterization of the lipid fraction of a pressurized ethanol extract of spirulina 10 platensis microalga is carried out. The list of cosmetic products below is a selection of the most requested cosmetics that contain the ingredient spirulina platensis extract. Cultivation of two species of spirulina spirulina platensis. The pharmacological actions, such as antiinflammation, antioxidation, antitumor, immunological enhancement, and hepatorenal protection of cphycocyanin cpc from spirulina platensis, have been reported, and cpc has important value of development and. Gelation capacity of spirulina platensis protein concentrate. Phycocyanin extraction from spirulina platensis and extract.
Phycocyanin extraction from cyanobacteria spirulina platensis was optimized using factorial design and response surface techniques. Spirulina and other bluegreen algae contain cphycocyanin, which acts as an accessory pigment when light energy is captured and transferred to chlorophyll a. The phycocyanin extract in the above method was prepared using three different methods. Phyocyanin extraction from microalgae spirulina platensis. Spirulina phycobiliproteins as food components and complements. Sternvolmer quenching constant value for zniicpc is determined. Influence of temperature on growth and biochemical. The extractions were carried out using six different methods, including chemical organic and inorganic acid treatment, physical freezing and thawing, sonication, homogenization and enzymatic lysozyme treatment methods. Medical application of spirulina platensis derived cphycocyanin. Administration of the hotwater extract of spirulina platensis enhanced the immune response of. Sarada et alprocess biochemistry 34 1999 795801 797 table 2 comparison of different methods for phycocyanin extraction from wet cells of spirulina method amount of phycocyanin extracted expressed in mg phycocyanin from 100 mg dry biomassa 1st extraction 3rd extraction total2nd extraction treated control treated controlcontrol treated control treated. Optimization of phycocyanin extraction from spirulina.
Optimization of phycocyanin extraction from spirulina platensis. Jan 16, 20 a method for stably purifying a functional dye, phycocyanin from spirulina platensis was developed by a hexane extraction process combined with high pressure. Phycocyanin extraction from spirulina platensis and extract stability under various ph and temperature article pdf available january 2009 with 1,485 reads how we measure reads. Stable isolation of phycocyanin from spirulina platensis. Extraction using sonication by water proved to be an efficient method. Optimization of protein extraction from spirulina platensis. Quantitative analysis of cphycocyanin from spirulina pacifica low temperature method background. In this study a simple protocol was developed for purifying phycocyanin pc from spirulina platensis ccc540 by using ammonium sulphate precipitation, followed by a single step chromatography by using deaecellulose11 and acetate buffer. A simple method for efficient extraction and separation of.
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